We are professionals in applying phage display technology to generate protein, antibody libraries for the develop and antibody discovery purposes. Our services involve the construction and screening of custom phage display libraries. In addition, we also provide a series of phage display based services.

Phage display is mainly based on the use of filamentous phage which is a kind of virus lives on Escherichia coli and has been proven as a powerful method to interrogate libraries containing millions or even billions of different peptides or proteins. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype. Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. To detect the interaction between displayed protein and those other molecules, phage display library can be screened to against other proteins.

One of the most successful applications of phage display technology has been the isolation of specific antibodies. As the first proteins which is successfully displayed on the surface of phage, antibody phage display is achieved by fusing the coding sequence of scFv or Fab to the coat protein. Due to the smaller size, scFv libraries are genetically more stable than Fab libraries, while Fab libraries lack the tendency to form higher molecular weight species, such as dimers and trimers, which can simplify the selection and characterization. Through binding the specific target, the interest antibody can then be eluted and retrieved. In this way, we are able to obtain various kinds of novel antibodies, such as canine (dog) monoclonal antibodies and anti-idiotype antibodies.

With experience, our scientists have developed several phage display based platforms and tailored hundreds of particular libraries and thousands of specific antibody products to boost our global customers’ research and project goals. We are pleased to use our extensive experience and advanced platform to offer the best service and the most qualified products to satisfy each individual demand from our customers.