In vitro molecular optimization is a very efficient means of generating mutant proteins with improved or novel properties, identifying regulatory sequences, and probing for structurally and functionally critical residues. Mutant libraries constructed using the in vitro molecular optimization method provide a useful approach to the systematic study of protein properties, regulation, and function.

Truncation Libraries

Truncation technology incrementally trims genes around a defined core region. Genes can be truncated from both the 5′ and the 3′ ends simultaneously to produce an incremental truncation library. Unlike other methods, our process preserves the open reading frame, avoiding out-of- frame mutations.

Applications

• Optimize solubility
• Evaluate minimal functional-protein size
• Identify functional domains
• Screen for inhibitors
• Map epitopes

Advantages

• No system-based out-of-frame mutations
• Low ancillary mutation rates—>90% sequence integrity (depending on length of the gene;
• unintended mutants will have amino acid substitutions in most cases)
• High success rates—receive all possible truncation variants; library diversity of up to ~40,000